Key Points Importing qPCR data and experimental designs into R Use the skip argument to skip lines when reading in a text file. Plots can be used to inspect the contents of large data frames. The separate() fucntion from tidyr can be used to separate variables contained in a single column. Data frames can be combined based on matching variables. Analysing qPCR data There is a lot of data wrangling involved in data analysis! The group_by() and summarise() functions are useful for calculating means across groups of samples. The mutate() function can be used to store the results of calculations in a new column. Using R for analysis help make the analysis reproducible. Glossary FIXME